Overview of CIRES

CIRES (Correlation Index-based Responsive Enzyme gene Screening) is a method to predict regulatory enzyme gene(s) in the biosynthesis of glycan structure of interest. To achieve this, CIRES utilizes quantitative genotype-phenotype correlation (Yamamoto H, et al. (2007) PLoS ONE 2(11): e1305).

Here, transcriptomic expression data of the glycan-related genes (such as glycosyltransferase genes) is used for "genotype" and cell surface glycan expression is used for "phenotype". Cross-sample comparisons are performed by calculating Pearson's correlation coefficients between gene expression profiles and glycan expression profiles.

Here is an example of GL7 epitope. GL7 is a rat monoclonal antibody, whose epitope turned out to be α2,6-linked Neu5Gc (Naito Y, et al. (2007) MCB, 27(8): 3008).

Transcriptomic gene expression profiles can be reused over and over again thus this database was developed. User only need to prepare glycan expression profiles to the CIRES correlation calculation and the database will calcula how much each gene(s) expression profile is sumilar or dissimilar to the glycan expression profile among choice of cells as correlation index. individual glycan-related genes.